PONCEU STAIN S COLOR SOLUTION 1473
PONCEU STAIN S COLOR SOLUTION
S is a rapid and reversible staining method used for the detection of protein bands on Western blot membranes, Polyvinylidene fluoride (PVDF), nitrocellulose, and cellulose acetate membranes. Ponceau Stain S is a negative stain that binds to the positively charged functional groups of the protein (amino group) and the non‐polar regions of the protein. The method could also be used to measure the microgram quantities of transferred protein by obtaining the reddish pink protein bands with a clear background. The Ponceau Stain S is reversible; this quality makes it useful for further immunological detection.
Ponceau Stain S is useful to locate and identify the proteins transferred electrophoretic ally onto nitrocellulose/nylon membranes before the antibody-mediated detection as the dye does not interfere with antibody detection in Western blots.
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